Both Rossi and Lorenzo intend to carry the fight to the flashy and fearless rookie Marquez in the 28 lap race.
The trio share the front row of the grid with Lorenzo (second) and Rossi (third) planning instant attacks on Marquez’s stunning pace.
Marquez claimed pole position with a massive 0.5s gap over the Yamaha pair.
Always inspired by racing at home Rossi said his return to strong qualifying form will be the platform for his podium challenge.
Misano is Rossi’s first front row start since the US Grand Prix at Laguna Seca in July.
“I am very satisfied with this qualifying, usually I suffer in this session,” Rossi said.
“The bike was fantastic with new tires, I could push a lot especially with the second one for my fast lap.
“Starting on the front row is very important here because the two Hondas are very fast as is Jorge.
“I want to arrive on the podium so I want to go with them early in the race. I like the new seamless gearbox a lot, it is very precise.”
Lorenzo feels that the 0.5s gap in qualifying is not a true reflection of race pace and that the introduction of Yamaha’s slick-shifting seamless gearbox will be a boost.
Lorenzo expects that improved rear tyre life over the race distance will be one significant advantage of the new gearbox.
Marquez shrugged off a crash in the final practice session before staging his impressive qualifying performance.
He admitted to a “stupid mistake” as the cause of the crash and also confirmed that his advantage for pole position is “not the real gap” to Lorenzo and Rossi. Nor his teammate Dani Pedrosa who qualified fourth.
“I made a stupid mistake with the crash,” Marquez said.
“When I released the front brake in the very last part of braking I started to open the gas and I just touched my finger on the brake lever which caused me to lose the front.
“The most important thing is the race rhythm and Jorge, Valentino and Dani have good pace. Plus you cannot win the championship in qualifying, only the race.”
Rossi will use a special helmet design – a tribute to his great friend Marco Simoncelli – in the race.
The Misano Circuit is named in honor of Simoncelli who was killed in a race crash at Sepang in the 2011 Malaysian Grand Prix.
“It is a long time since Marco has not been with us and this was his own track, more than mine,” Rossi said.
“His house was closer to the circuit than mine at Tavullia so I decided on a helmet design with a song from Pink Floyd that reminds me of Marco.”